Human Masseter Muscle Fibers From the Elderly Express Less Neonatal Myosin Than Those of Young Adults
Identifieur interne : 003A64 ( Main/Exploration ); précédent : 003A63; suivant : 003A65Human Masseter Muscle Fibers From the Elderly Express Less Neonatal Myosin Than Those of Young Adults
Auteurs : Erika Cvetko [Slovénie] ; Pet Karen ; Ji Janá Ek ; Lucie Kubínová ; Ana L Pez Plasencia [Espagne] ; Ida Eržen [Slovénie]Source :
- The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology [ 1932-8486 ] ; 2012-08.
English descriptors
- KwdEn :
- Adult myhc isoforms, Adult subjects, Area density, Area proportion, Biochem biophys, Biol, Coexpressed, Coexpression, Confocal, Confocal microscopy, Cvetko, Czech republic, Developmental myhc isoforms, Different myhc isoforms, Eijden, Eijden tmgj, Elderly groups, Elderly subjects, Elderly subjects years, Ellipse program, Fiber, Fiber diameter, Fiber type, Fiber type composition, Fiber types, Fibers coexpressing, Fibre, Functional significance, Gene expression, Grant number, Grant sponsor, Human masseter, Human masseter muscle, Human masticatory muscles, Hybrid fibers, Image analysis, Immunoperoxidase method, Individual fibers, Isoform, Isoforms, Large sample, Limb muscles, Line projection, Lower area proportion, Lower regeneration capacity, Masseter, Masseter muscle, Masseter muscle table, Masseter muscles, Masticatory, Mech ageing, Monemi, Monoclonal antibodies, Multipennate muscle, Muscle fiber types, Muscle fibers, Muscle sample, Muscle specimens, Myhc, Myhc expression, Myhc isoform expression, Myhc isoforms, Myosin, Neonatal myosin, Numerical proportion, Particular myhc isoform, Particular myhc isoforms, Previous studies, Pure fibers, Relative amount, Sarcomere length, Several fibers, Short fibers, Significant differences, Slow muscle, Smaller proportion, Substantial portion, Vascular supply, Wiley periodicals, Young adults, Young masseter muscle, Young subjects.
- Teeft :
- Adult myhc isoforms, Adult subjects, Area density, Area proportion, Biochem biophys, Biol, Coexpressed, Coexpression, Confocal, Confocal microscopy, Cvetko, Czech republic, Developmental myhc isoforms, Different myhc isoforms, Eijden, Eijden tmgj, Elderly groups, Elderly subjects, Elderly subjects years, Ellipse program, Fiber, Fiber diameter, Fiber type, Fiber type composition, Fiber types, Fibers coexpressing, Fibre, Functional significance, Gene expression, Grant number, Grant sponsor, Human masseter, Human masseter muscle, Human masticatory muscles, Hybrid fibers, Image analysis, Immunoperoxidase method, Individual fibers, Isoform, Isoforms, Large sample, Limb muscles, Line projection, Lower area proportion, Lower regeneration capacity, Masseter, Masseter muscle, Masseter muscle table, Masseter muscles, Masticatory, Mech ageing, Monemi, Monoclonal antibodies, Multipennate muscle, Muscle fiber types, Muscle fibers, Muscle sample, Muscle specimens, Myhc, Myhc expression, Myhc isoform expression, Myhc isoforms, Myosin, Neonatal myosin, Numerical proportion, Particular myhc isoform, Particular myhc isoforms, Previous studies, Pure fibers, Relative amount, Sarcomere length, Several fibers, Short fibers, Significant differences, Slow muscle, Smaller proportion, Substantial portion, Vascular supply, Wiley periodicals, Young adults, Young masseter muscle, Young subjects.
Abstract
In contrast to limb muscles where neonatal myosin (MyHC‐neo) is present only shortly after birth, adult masseter muscles contain a substantial portion of MyHC‐neo, which is coexpressed with mature MyHC isoforms. Changes in the numerical and area proportion of muscle fibers containing MyHC‐neo in masseter muscle with aging could be expected, based on previously reported findings that (i) developmental MyHC‐containing muscle fibers exhibit lower shortening velocities compared to fibers with exclusively fast MyHC isoforms and (ii) transformation toward faster phenotype occurs in elderly compared to young masseter muscle. In this study, we detected MyHC isoforms in the anterior superficial part of the human masseter muscle in a sufficiently large sample of young, middle‐aged, and elderly subjects to reveal age‐related changes in the coexpression of MyHC‐neo with adult MyHC isoforms. MyHC isoforms were visualized with immunoperoxidase method and the results were presented by (i) the area proportion of fibers containing particular MyHC isoforms and (ii) the numerical proportion of fiber types defined by MyHC‐1, ‐2a, ‐2x, and ‐neonatal isoform expression from a successive transverse sections. We found a lower numerical and area proportion of fibers expressing MyHC‐neo as well as a lower area proportion of fibers containing MyHC‐1 in elderly than in young subjects. We conclude that the diminished expression of MyHC‐neo with age could point to a lower regeneration capacity of masseter muscle in the elderly. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/ar.22512
Affiliations:
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Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2012-08">2012-08</date></imprint><idno type="ISSN">1932-8486</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1932-8486</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult myhc isoforms</term><term>Adult subjects</term><term>Area density</term><term>Area proportion</term><term>Biochem biophys</term><term>Biol</term><term>Coexpressed</term><term>Coexpression</term><term>Confocal</term><term>Confocal microscopy</term><term>Cvetko</term><term>Czech republic</term><term>Developmental myhc isoforms</term><term>Different myhc isoforms</term><term>Eijden</term><term>Eijden tmgj</term><term>Elderly groups</term><term>Elderly subjects</term><term>Elderly subjects years</term><term>Ellipse program</term><term>Fiber</term><term>Fiber diameter</term><term>Fiber type</term><term>Fiber type composition</term><term>Fiber types</term><term>Fibers coexpressing</term><term>Fibre</term><term>Functional significance</term><term>Gene expression</term><term>Grant number</term><term>Grant sponsor</term><term>Human masseter</term><term>Human masseter muscle</term><term>Human masticatory muscles</term><term>Hybrid fibers</term><term>Image analysis</term><term>Immunoperoxidase method</term><term>Individual fibers</term><term>Isoform</term><term>Isoforms</term><term>Large sample</term><term>Limb muscles</term><term>Line projection</term><term>Lower area proportion</term><term>Lower regeneration capacity</term><term>Masseter</term><term>Masseter muscle</term><term>Masseter muscle table</term><term>Masseter muscles</term><term>Masticatory</term><term>Mech ageing</term><term>Monemi</term><term>Monoclonal antibodies</term><term>Multipennate muscle</term><term>Muscle fiber types</term><term>Muscle fibers</term><term>Muscle sample</term><term>Muscle specimens</term><term>Myhc</term><term>Myhc expression</term><term>Myhc isoform expression</term><term>Myhc isoforms</term><term>Myosin</term><term>Neonatal myosin</term><term>Numerical proportion</term><term>Particular myhc isoform</term><term>Particular myhc isoforms</term><term>Previous studies</term><term>Pure fibers</term><term>Relative amount</term><term>Sarcomere length</term><term>Several fibers</term><term>Short fibers</term><term>Significant differences</term><term>Slow muscle</term><term>Smaller proportion</term><term>Substantial portion</term><term>Vascular supply</term><term>Wiley periodicals</term><term>Young adults</term><term>Young masseter muscle</term><term>Young subjects</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adult myhc isoforms</term><term>Adult subjects</term><term>Area density</term><term>Area proportion</term><term>Biochem biophys</term><term>Biol</term><term>Coexpressed</term><term>Coexpression</term><term>Confocal</term><term>Confocal microscopy</term><term>Cvetko</term><term>Czech republic</term><term>Developmental myhc isoforms</term><term>Different myhc isoforms</term><term>Eijden</term><term>Eijden tmgj</term><term>Elderly groups</term><term>Elderly subjects</term><term>Elderly subjects years</term><term>Ellipse program</term><term>Fiber</term><term>Fiber diameter</term><term>Fiber type</term><term>Fiber type composition</term><term>Fiber types</term><term>Fibers coexpressing</term><term>Fibre</term><term>Functional significance</term><term>Gene expression</term><term>Grant number</term><term>Grant sponsor</term><term>Human masseter</term><term>Human masseter muscle</term><term>Human masticatory muscles</term><term>Hybrid fibers</term><term>Image analysis</term><term>Immunoperoxidase method</term><term>Individual fibers</term><term>Isoform</term><term>Isoforms</term><term>Large sample</term><term>Limb muscles</term><term>Line projection</term><term>Lower area proportion</term><term>Lower regeneration capacity</term><term>Masseter</term><term>Masseter muscle</term><term>Masseter muscle table</term><term>Masseter muscles</term><term>Masticatory</term><term>Mech ageing</term><term>Monemi</term><term>Monoclonal antibodies</term><term>Multipennate muscle</term><term>Muscle fiber types</term><term>Muscle fibers</term><term>Muscle sample</term><term>Muscle specimens</term><term>Myhc</term><term>Myhc expression</term><term>Myhc isoform expression</term><term>Myhc isoforms</term><term>Myosin</term><term>Neonatal myosin</term><term>Numerical proportion</term><term>Particular myhc isoform</term><term>Particular myhc isoforms</term><term>Previous studies</term><term>Pure fibers</term><term>Relative amount</term><term>Sarcomere length</term><term>Several fibers</term><term>Short fibers</term><term>Significant differences</term><term>Slow muscle</term><term>Smaller proportion</term><term>Substantial portion</term><term>Vascular supply</term><term>Wiley periodicals</term><term>Young adults</term><term>Young masseter muscle</term><term>Young subjects</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In contrast to limb muscles where neonatal myosin (MyHC‐neo) is present only shortly after birth, adult masseter muscles contain a substantial portion of MyHC‐neo, which is coexpressed with mature MyHC isoforms. Changes in the numerical and area proportion of muscle fibers containing MyHC‐neo in masseter muscle with aging could be expected, based on previously reported findings that (i) developmental MyHC‐containing muscle fibers exhibit lower shortening velocities compared to fibers with exclusively fast MyHC isoforms and (ii) transformation toward faster phenotype occurs in elderly compared to young masseter muscle. In this study, we detected MyHC isoforms in the anterior superficial part of the human masseter muscle in a sufficiently large sample of young, middle‐aged, and elderly subjects to reveal age‐related changes in the coexpression of MyHC‐neo with adult MyHC isoforms. MyHC isoforms were visualized with immunoperoxidase method and the results were presented by (i) the area proportion of fibers containing particular MyHC isoforms and (ii) the numerical proportion of fiber types defined by MyHC‐1, ‐2a, ‐2x, and ‐neonatal isoform expression from a successive transverse sections. We found a lower numerical and area proportion of fibers expressing MyHC‐neo as well as a lower area proportion of fibers containing MyHC‐1 in elderly than in young subjects. We conclude that the diminished expression of MyHC‐neo with age could point to a lower regeneration capacity of masseter muscle in the elderly. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>Slovénie</li></country></list><tree><noCountry><name sortKey="Jana Ek, Ji" sort="Jana Ek, Ji" uniqKey="Jana Ek J" first="Ji" last="Janá Ek">Ji Janá Ek</name><name sortKey="Karen, Pet" sort="Karen, Pet" uniqKey="Karen P" first="Pet" last="Karen">Pet Karen</name><name sortKey="Kubinova, Lucie" sort="Kubinova, Lucie" uniqKey="Kubinova L" first="Lucie" last="Kubínová">Lucie Kubínová</name></noCountry><country name="Slovénie"><noRegion><name sortKey="Cvetko, Erika" sort="Cvetko, Erika" uniqKey="Cvetko E" first="Erika" last="Cvetko">Erika Cvetko</name></noRegion><name sortKey="Cvetko, Erika" sort="Cvetko, Erika" uniqKey="Cvetko E" first="Erika" last="Cvetko">Erika Cvetko</name><name sortKey="Cvetko, Erika" sort="Cvetko, Erika" uniqKey="Cvetko E" first="Erika" last="Cvetko">Erika Cvetko</name><name sortKey="Erzen, Ida" sort="Erzen, Ida" uniqKey="Erzen I" first="Ida" last="Eržen">Ida Eržen</name></country><country name="Espagne"><noRegion><name sortKey="Plasencia, Ana L Pez" sort="Plasencia, Ana L Pez" uniqKey="Plasencia A" first="Ana L Pez" last="Plasencia">Ana L Pez Plasencia</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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